Additional file 11.
Expression of duplicate F3'5'Hs in berry skin of four cultivars accumulating 3'5'-OH anthocyanins detected by semiquantitative PCR. Berry skin was sampled at four developmental stages. cDNA was normalised using the housekeeping Ubiquitin gene. UFGT was used as a marker for anthocyanin gene expression. Even though the pre-veraison berries were sampled over green bunches immediately before visible colour transition, expression of UFGT had already been triggered in 'Aglianico' and was barely detectable in 'Nebbiolo'. Either primer of the oligonucleotide pairs targeting the F3'5'Hi and -l copies anneals to either exon of the corresponding gene model. The corresponding PCR bands obtained from gDNA are approximately 400 bp longer than the cDNA amplicons shown in the stripes of the electrophoresis gel of this figure.
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Falginella et al. BMC Genomics 2010 11:562 doi:10.1186/1471-2164-11-562