Sequence and transcriptional analysis of miRNAs dominantly expressed in S. japonicum. A. Alignment of sja-mir-7 sequences with homologues from other organisms. The seed sequences are shadowed in a dark colour. B. Northern-blot hybridization using probes complementary to the dominant miRNAs expressed during the adult worm stage. The 70 nt pre-miRNAs were indicated by arrows. C. Quantification analysis, relative to that in the egg stage, of miRNAs dominantly expressed in four developmental stages as indicated in different colours.
Hao et al. BMC Genomics 2010 11:55 doi:10.1186/1471-2164-11-55