Additional file 1.
Figure S1: Expression of the CREB and CREMτ proteins in adult mouse testis.A. Immunofluoresence on sections from adult mouse testis with the anti-CREB and CREMτ antibodies. The signal for the antibodies in red, the Hoechst stained nuclei and the merged views are show as indicated. Representative examples of the different cell types are indicated by arrows. L; Leydig cells, S; Sertoli cells; Sp; spermatogonia; P; pachytene spermatocytes, I; intertubular cells, R; round spermatids, E; elongated spermatids. These results reveal a distinct expression of CREB and CREMτ in the testis, where CREB is present in the spermatogonia, the Sertoli, Leydig and intertubular cells, while CREMτ is present in haploid round spermatids. 20 fold magnification. B. Western blots with the CREMτ and CREB antibodies on 20 ug of extracts from the indicated cells or tissues. ES is undifferentiated mouse E14 embryonic stem cells, COS is COS-1, and 3T3 is NIH3T3. The CREB antibody detects a single polypeptide corresponding to CREB in each of the extracts, while the CREMτ antibody detects the CREMτ proteins only in the testis extract.
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Martianov et al. BMC Genomics 2010 11:530 doi:10.1186/1471-2164-11-530