Immuno-fluorescent staining for expression of stem cell molecules on ocular surface epithelium. Immunofluorescence with HES1 and FRZB1 was performed on radial cut sections of LEC (A, B, K, L), limbus (C, D, M, N) cornea (E, F, O, P) and tonsil (G, H, Q, R). Images (K, L, G, H, Q, R, I, J) are at 40× magnification, the remainder are at 20× magnification (scale bars shown). HES1 immunofluorescence co-localised with DAPI nuclear dye is shown in images (B. D, F, H,). Images (A, C, E, G) show HES1 (TRITC) staining. FRZB1 immunofluorescence co-localised with DAPI dye is shown in images (L, N, P, R). Images (K, M, O, Q,) are of FRZB1 with TRITC dye. Positive staining for HES1 and FRZB1 (B, D, F, H), (N, P, R) is seen as whitish nuclear stain, due to co-localisation of FRZB1 and HES1 (TRITC dye) and nuclear stain DAPI in the cell nuclei. FRZB1 immunofluorescence of LEC (L) is seen as yellow colouration as it intensely stained the nuclei of the basal epithelial cells in the LEC and masked the underlying DAPI staining. Positive nuclear staining of Tonsil with HES1 antibody (G, H) has speckled appearance and FRZB1 (Q, R) appears as a rim around the cell nucleus. Images (I, J) and (S, T) are negative controls for HES1 and FRZB1 antibody respectively.
Kulkarni et al. BMC Genomics 2010 11:526 doi:10.1186/1471-2164-11-526