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Open Access Highly Accessed Research article

Deep sequencing of small RNA libraries reveals dynamic regulation of conserved and novel microRNAs and microRNA-stars during silkworm development

Guru Jagadeeswaran1, Yun Zheng2, Niranji Sumathipala3, Haobo Jiang3, Estela L Arrese1, Jose L Soulages1, Weixiong Zhang24 and Ramanjulu Sunkar1*

Author affiliations

1 Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA

2 Department of Computer Science and Engineering, Washington University in St. Louis, St Louis MO 63130, USA

3 Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, USA

4 Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA

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Citation and License

BMC Genomics 2010, 11:52  doi:10.1186/1471-2164-11-52

Published: 20 January 2010

Abstract

Background

In eukaryotes, microRNAs (miRNAs) have emerged as critical regulators of gene expression. The Silkworm (Bombyx mori L.) is one of the most suitable lepidopteran insects for studying the molecular aspects of metamorphosis because of its large size, availability of mutants and genome sequence. Besides, this insect also has been amply studied from a physiological and biochemical perspective. Deep sequencing of small RNAs isolated from different stages of silkworm is a powerful tool not only for measuring the changes in miRNA profile but also for discovering novel miRNAs.

Results

We generated small RNA libraries from feeding larvae, spinning larvae, pupae and adults of B. mori and obtained ~2.5 million reads of 18-30 nt. Sequence analysis identified 14 novel and 101 conserved miRNAs. Most novel miRNAs are preferentially expressed in pupae, whereas more than 95% of the conserved miRNAs are dynamically regulated during different developmental stages. Remarkably, the miRNA-star (miR*) of four miRNAs are expressed at much higher levels than their corresponding miRNAs, and their expression profiles are distinct from their corresponding miRNA profiles during different developmental stages. Additionally, we detected two antisense miRNA loci (miR-263-S and miR-263-AS; miR-306-S and miR-306-AS) that are expressed in sense and antisense directions. Interestingly, miR-263 and miR-306 are preferentially and abundantly expressed in pupae and adults, respectively.

Conclusions

We identified 101 homologs of conserved miRNAs, 14 species-specific and two antisense miRNAs in the silkworm. Our results provided deeper insights into changes in conserved and novel miRNA and miRNA* accumulation during development.