Additional file 5.

Validation of Affymetrix microarray hybridization results by quantitative RT-PCR. Expression levels were converted to theoretical value R0 using the formula R0 = R(Ct) (1 + E)(-Ct), where R0 is the starting fluorescence, R(Ct) is the fluorescence at the threshold cycle Ct and E is the amplification efficiency. The R0 values were normalized to R0 of actin gene followed by log-transformation. The expression levels in SN and 1:1 mixture of AT and TC RNA were compared using the t-test.

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Akhunova et al. BMC Genomics 2010 11:505   doi:10.1186/1471-2164-11-505