Amplification of mtDNA and cDNA LSU rRNA gene regions in Crassostrea virginica. Diagram A presents the amplicon locations in the C. virginica and A. irradians mtDNA genome. Gel image B contains a 233 bp region of the 5'-half LSU amplified from the C. virginica genomic DNA preparation (lane 2) and cDNA preparation (lane 3), as well as a 494 bp region of the 3'-half LSU amplified from the C. virginica genomic DNA preparation (lane 4) and cDNA preparation (lane 5); lane 1 = 100 bp ladder. Gel image C: a 748 bp product (starting in the 5' half and ending in the 3' half) amplified from a continuous LSU template in Argopecten irradians genomic DNA (lane 2) and cDNA (lane 3), but was not amplified from C. virginica genomic DNA (lane 4) or cDNA (lane 5).
Milbury et al. BMC Genomics 2010 11:485 doi:10.1186/1471-2164-11-485