Open Access Research article

Combination of genomic approaches with functional genetic experiments reveals two modes of repression of yeast middle-phase meiosis genes

Michael Klutstein123, Zahava Siegfried1, Ariel Gispan1, Shlomit Farkash-Amar1, Guy Zinman4, Ziv Bar-Joseph4, Giora Simchen2* and Itamar Simon1*

Author Affiliations

1 Department of Microbiology and Molecular Genetics, The Institute for Medical Research - Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, 91120 Israel

2 Department of Genetics, The Hebrew University, Jerusalem, 91904 Israel

3 Telomere Biology Laboratory, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3PX, UK

4 School of Computer Science, Carnegie Mellon University, Pittsburgh, PA 15213, USA

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BMC Genomics 2010, 11:478  doi:10.1186/1471-2164-11-478

Published: 17 August 2010

Additional files

Additional file 1:

Genomic processed data. The file contains all the genomic (ChIP-chip and expression) data in the different genetic backgrounds, as well as some filters applied to the data, and some validation data from other sources (indicated).

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Additional file 2:

Validation of microarray data by RT-PCR. The file contains RT-PCR data on several transcripts in several genetic backgrounds and time points (indicated).

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Additional file 3:

Genes in 3 binding groups. The file contains the Y names of the genes in the Ndt80-only, Sum1-only and Common binding groups.

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Additional file 4:

Validation by ChIP-PCR of selected targets. The file contains ChIP-PCR results on several promoter regions (indicated).

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Additional file 5:

NDT80 accumulates in ER strain. The file contains RT-PCR data on the NDT80 transcript in WT and ER strains. Shows constitutive expression of NDT80 in the presence of Estradiol.

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Additional file 6:

Kinetics of meiosis in the MK-ER-Ndt80 strain. The file contains nuclei counting results after DAPI staining in different time points after Estradiol addition to the medium.

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Additional file 7:

Ndt80 repression metric calculated for every common target gene. The file contains the calculated metric for the contribution of the indirect regulation of Ndt80 to the repression of every common target gene of Ndt80 and Sum1.

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Additional file 8:

Sum1 activity during meiosis. The file contains Western Blot of Sum1-myc during W303 meiosis.

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Additional file 9:

Strains used in this study. The file contains the genotypes of all strains used in this study.

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Additional file 10:

Kinetics of meiosis in WT and sum1-deletion strains. The file contains nuclei counting results after DAPI staining of WT and sum1-deletion strains during meiosis.

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