Table 5

Validation of microarray and RNA sequencing of yeast grown in nitrogen poor (MM) and nitrogen rich (SC) conditions using Real Time RT PCR.

Microarray

RNA sequencing

RT PCR

Gene ID

MTP

oligo(dT)

MTP

oligo(dT)

MTP

Oligo(dT)

Ratio MM/SC

p value

Ratio MM/SC

p value

Ratio MM/SC

p value

Ratio MM/SC

p value

Ratio MM/SC

Ratio MM/SC


YDL244W

1.98

0.00

3.16

0.11

28.94

39.62

YGR225W

1.72

0.00

7.65

0.00

4.08

3.13

YGR249W

1.66

0.01

5.80

0.00

4.79

8.20

YMR025W

1.51

0.00

3.96

0.15

2.10

1.57

YNR072W

-1.24

0.05

-6.32

0.00

-2.34

-1.36

YFR034C

-1.42

0.02

-1.25

0.10

-9.08

0.00

-6.41

0.08

1.30

1.24

YKR053C

-5.37

0.00

-1.88

0.14

-2.16

0.00

1.15

0.51

-2.10

-2.05

YLR126C

1.54

0.00

1.36

0.11

5.54

0.05

1.40

0.42

1.58

1.95

YMR003W

1.25

0.01

1.11

0.26

7.91

0.01

2.65

0.08

1.03

1.95

YOR252W

1.29

0.01

1.03

0.46

6.33

0.00

1.54

0.10

1.99

1.54


Adomas et al. BMC Genomics 2010 11:477   doi:10.1186/1471-2164-11-477

Open Data