Table 1

Overview of the results comparing use of oligo(dT) and multi-targeted primers (MTPs) for reverse transcription.

Saccharomyces cerevisiae

Neurospora crassa

Feature

Nitrogen depletion

Protoperithecial developmenr


Oligo (dT)

MTP

Common

Oligo (dT)

MTP

Common


Number of well measured genes1 on the array

4620

6042

4573

469

416

Number of genes significantly differentially expressed (P ≤ 0.05)

2155

3577

1839

172

406

2021

Number of up-regulated genes (P ≤ 0.05)

1080

1855

925

66

232

31

Number of down-regulated genes (P ≤ 0.05)

1075

1722

912

106

174

61

Highest gene expression ratio

49.15

32.6

24.26

10.74

Average gene expression ratio

2.05

1.77

1.96

1.43

GEL502

1.51

1.27

1.91

2.08

Number of genes with gene expression ratio greater or equal to GEL50 (P ≤ 0.05)

1237

2617

1166

102

141

52


1 well measured genes recorded after data normalization (foreground fluorescence signals were at least three standard deviations of the distribution of intensities of the background pixels for that gene).

2 GEL50 - gene expression level at which there is a 50% empirical probability of a significant call.

Adomas et al. BMC Genomics 2010 11:477   doi:10.1186/1471-2164-11-477

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