Table 1

Overview of the phenotypes associated with the different prosV alleles.

Allele

Genotype

Stage of lethality

Larval taste response

Pros expression in AMC

Pros expression in CNS

Axonal routing in AMC


V14

Wild type (complete PGal4 remobilization)

Viable

Normal

Normal

Normal

Normal


V13

Partial pGal4 excision (remaining of 718 bp)

Young adult < 2 days old

Normal

Normal

altered

Normal


V24

Partial pGal4 excision (remaining of 7400 pb)

pupal

Intermediate

Normal

altered

Normal


V1

full length pGal4 transposon (12900 pb)

larva

Altered

absent

altered

Misrouting


(redrawn from Guenin et al. [8]).

In the prosV1 (V1) allele, the full length PGal4 transposon is inserted upstream of the pros coding region (-216 bp). prosV14 (V14) results from the correct and total remobilization of the transposon, in this strain the wild type phenotype is restored. In prosV24 (V24) and prosV13 (V13), the PGal4 element has been partially removed, respectively 7400 and 718 bp remain inserted 216 bp upstream the pros start site. The peak of developmental lethality, the taste response of late homozygous 2nd instar larva, Pros expression level in larvae and axonal misrouting are indicated for each prosV allele.

The larval taste response was measured towards 0.1 M sucrose and 0.3 M NaCl concentration that are known to respectively attract or repulse wild type Drosophila. V1 mutants were indifferent to both substances (altered taste response), V24 showed an intermediate response: they were repulsed by NaCl but remained indifferent to sucrose and V13 and V14 present a normal taste response to both substances. The Pros expression pattern is indicated by comparison to the V14 wild type: In the AMC, V1 showed no Pros expression but for the other alleles, Pros expression pattern was similar to V14. In the CNS, all mutant alleles showed a distinct altered expression pattern as compared to the wild type (further descriptions of the Pros pattern are found in the text and in [Additional file 2]).

Guenin et al. BMC Genomics 2010 11:47   doi:10.1186/1471-2164-11-47

Open Data