Gene expression analyses. (A) Hierarchical clustering of 5950 genes for a total of 17 samples relative to the larval AMC and CNS of the different prosV mutants. Each row represents a gene and each column a sample. For each organ, the samples at the top of the image are classified according to the severity of their phenotypes (from wild type to the most severe phenotype: V14, V13, V24 and V1). Each cell in the matrix corresponds to the expression level of one gene in a sample (see colour scale at the bottom of the image). The yellow frames represent the AMC tissue specific signature and contain the genes that are differentially expressed between AMC and the CNS independently of Pros expression. (B) Discriminating scores (DS) smoothed in a window of 100 genes, calculated between V1 and other prosV in the AMC (in red), and between V1 and V14 in the CNS (in blue), among the gene clusters. In the AMC, three peaks, annotated 1, 2 and 3 (black bars), appear to be enriched in differentially expressed genes, they have been associated respectively to "cell fate commitment", "proteasome complex" and "signal transduction" ontologies. (C) Hierarchical clustering of the 306 genes present in peak 1 in the AMC. Pink frame zooms on a set of highly correlated (r>0.9) genes that are differentially expressed between V1 and all other alleles in the AMC. These genes are referenced on the right according to the Drosophila nomenclature (see also Table 3). The dendrogram on the left represents correlation distances between the profiles of the studied genes. Differentially expressed genes indicated in red were common with CNS. (D) Same as (C) in CNS samples. The Pink framed region contains 86 genes which are referenced on the right according to the Drosophila nomenclature. Differentially expressed genes indicated in red were common with AMC. (E) Motif found in the promoting region of the 28 genes common to AMC and CNS (genes indicated in red).
Guenin et al. BMC Genomics 2010 11:47 doi:10.1186/1471-2164-11-47