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Open Access Research article

Expression profiling of prospero in the Drosophila larval chemosensory organ: Between growth and outgrowth

Laure Guenin12, Mahatsangy Raharijaona34, Rémi Houlgatte34 and Fawzia Baba-Aissa2*

Author Affiliations

1 Institut Pasteur, Pathogénomique Mycobactérienne Intégrée, 25, Rue du Dr. Roux, 75724 Paris Cedex 15, France

2 Université de Bourgogne, Facultés des Sciences, Unité Mixte de Recherche 5548 Associée au Centre National de la Recherche Scientifique, 6, Bd Gabriel, 21 000 Dijon, France

3 INSERM, U915, Nantes, F-44000, France

4 Université de Nantes, l'Institut du Thorax, Nantes, F-44000, France

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BMC Genomics 2010, 11:47  doi:10.1186/1471-2164-11-47

Published: 19 January 2010

Additional files

Additional file 1:

Mitotic Activity in the AMC region, in stage 11 and stage 16 embryos. Wild type embryos from stage 11 to 16 were stained with Pros (red) and H3p (green) which label cells in division. (A) Mitotic activity is observed until stage 11-12 (arrow) while no more activity is detected from stage 16 embryos (B) in the AMC. Scale bar correspond to 20 μm.

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Additional file 2:

Expression pattern of V24 in the third instar larval CNS. The Pros (A) and Elav (B) expression pattern as well as the mitotic activity (C) of V24 are shown as compared to the previously reported pattern of V14, V13 and V1 alleles [8]. Although V24 and V13 present the same expression pattern in the AMC, the situation is different in the CNS. As it can be seen Pros (A) and Elav labeling (B) are distinctive for both alleles in the region delimiting the two hemispheres and the Optic lobes (OLs). As compared to V13, V24 presents a decrease of the staining in this region for these two markers (B). In ventral nerve cord (VNC), V13 shows an important hyperplasia (arrowheads) due to an excess of neurons. In V24, the VNC extremity presents a bifida aspect (arrow). The mitotic activity, revealed by anti-pHistone-H3 (H3p) (C) is strongly increased in V24, especially in the OLs.

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Additional file 3:

List of the AMC genes represented in the 3 peaks obtained after the application of the discriminating score between V1 and all other alleles for the AMC samples. Peak 1: Differentially expressed genes involved in cell fate commitment. Peak 2: Differentially expressed genes involved in the proteasome complex. Peak 3: Differentially expressed genes involved in signal transducer activity.

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Additional file 4:

List of the CNS genes represented in the peak obtained after the application of the discriminating score between V1 and V1 4 for the CNS samples. The peak contains differentially expressed genes involved in cell fate commitment.

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Additional file 5:

Supplemental Table S1: Genes found to be differentially expressed between V1 and V14 CNS. We found 86 genes that are highly correlated (coeff> 0,9). Among the 86 genes, 28 were also found to be overexpressed in the V1 AMC (column on the left, gene names are indicated in bold) and contain the common putative pros DNA motif in their promoter. The 58 genes present in the two columns on the right are specifically overexpressed in V1 CNS as compared to V14 CNS. Supplemental Table S2: Phenotypic data related to the candidate genes involved in neurite outgrowth and/or synaptic transmission. The criteria used for the description of the phenotypes were as follows: (1) if many larval phenotypes were available for a gene, we selected only those observed in the nervous system and preferentially in the peripheral nervous system (PNS). (2) If no larval phenotype was available for a gene, we selected those observed in the embryonic and/or adult PNS. (3) If available, the effect of the upregulation or downregulation of these genes is mentioned respectively for those that are overexpressed or underexpressed in V1 AMC. (4) All studies mentioned were done in Drosophila melanogaster. Supplemental Table S3: Phenotype data related to the candidate genes involved in growth and autophagy. The criteria used for the description of the phenotypes were the same as those for Table S2. Supplemental Table S4: Phenotype data related to the candidate genes involved in sensory organ development and most particularly in olfaction (in bold). The criteria used for the description of the phenotypes were the same as those for Table S2.

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