Table 2

In silico analysis of transcript bias in contigs formed from all sequences in each library, the ten highest R-values were analyzed using BLASTX (e-value ≤1E-10).

Library Bias

Contig #

Length (bp)

R1

p-value2

Identity

e-value

Identity (%)


Soldier

3

2647

106

5.24e-50

Viral Protein

1.00e-170

44


Soldier

794

1435

42

4.51e-25

Ejaculatory Bulb-specific Protein 3

1.00e-25

67


Alate

827

1036

31

1.42e-10

Ejaculatory Bulb-specific Protein 3

1.00e-29

73


Alate

148

981

66

6.63e-21

ATP Synthase Subunit A

1.00e-27

68


Alate

168

1724

26

1.03e-10

Cyctochrome C Oxidase Subunit 1

1.00e-180

86


Alate

352

1550

22

1.58e-11

Endoglucanase B Precursor (Cellulase B)

1.00e-109

59


Early Larval

472

954

92

3.61e-48

Cytochrome Oxidase Subunit 3

1.00e-86

76


Early Larval

479

1740

74

2.82e-35

Cytochrome B

1.00e-134

77


Early Larval

507

735

40

3.24e-20

Tropomyosin

1.00e-18

89


Early Larval

821

1349

18

7.46e-05

Troponin C

1.00e-38

70


Late Larval

412

975

80

3.10e-22

Ribosomal Protein L31

1.00e-51

83


1 R-statistic was based on equations in Stekel et al. (2000) and compared relative abundance of each libraries prevalence in contiguous sequences from all available sequences.

2 p-values were calculated to establish appropriate significance thresholds to confirm individual library abundance. p-value is in reference to significance of caste bias of singletons in contigs formed using a chi-squared test on singleton representation in each contig.

Steller et al. BMC Genomics 2010 11:463   doi:10.1186/1471-2164-11-463

Open Data