Expression profiles of selected mRNAs during newt heart regeneration. Expression of RNF 7, SFRP 1, Thioredoxin-like protein 4B and TCTP 1 was analysed by RT-PCR at 4, 7, 14 and 21 days after mechanical injury of newt ventricles (n = 3 for each time point). Expression of ribosomal protein S21 was analyzed as a non-modulated control. A more than 2 fold change in expression level was detected for all 4 selected genes during the newt heart regeneration. Statistically significant changes in expression (p < 0.05 by paired students t-test) were detected for RNF 7 and SFRP 1 at 14 and 21 days after injury and 7 and 14 days after injury for Thioredoxin-like protein 4B. Error bars are shown as ± STDEV. Please note that selected newt genes were so far not identified in regenerating zebrafish hearts.
Borchardt et al. BMC Genomics 2010 11:4 doi:10.1186/1471-2164-11-4