Additional file 9.
Recruitment of probable non-TOP mRNAs to polysomes in cardiomyocytes in response to ET-1 (Microsoft Excel Spreadsheet). Neonatal rat cardiomyocytes exposed to ET-1 (100 nM, 1 h) or left unstimulated (controls). Total and polysomal RNA were analysed using microarrays. Probesets corresponding to mRNAs with >1.25-fold increase in expression in polysomal RNA (but not total RNA) in response to ET-1 (FDR < 0.05, t-test with Benjamini -Hochberg false discovery rate correction) were identified. Mean raw fluorescence values are given for the controls with the mean fold change (n = 4) induced by insulin relative to controls. Mouse 5' UTR sequences up to and including the initiation codon are provided. Sequence identification used FANTOM3 and NCBI databases in addition to the database of transcriptional start sites (DBTSS). Presence (Y) or absence (N) of polypyrimidine or GC tracts, upstream open reading frames (uORFs), or 5' untranslated exons are indicated, in addition to GC content (%), UTR length and predicted minimum free energy (Mfe).
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Markou et al. BMC Genomics 2010 11:343 doi:10.1186/1471-2164-11-343