Additional file 5.
Regulation of polysomal and total RNA expression in cardiomyocytes in response to ET-1 (Microsoft Word Table). Neonatal rat cardiomyocytes were exposed to ET-1 (100 nM, 1 h) or left unstimulated (controls). Total and polysomal RNA were analysed using Affymetrix rat genome 230 2.0 microarrays. The data were normalised to controls. Transcripts with differential expression in ET-1-treated cells relative to controls were identified (>1.25-fold difference; * FDR < 0.05 ET-1 vs Controls for polysomal RNA, # FDR < 0.05 ET-1 vs Controls for total RNA, t-test with Benjamini and Hochberg false discovery rate correction). Mean raw fluorescence values are provided for controls, and mean expression relative to controls is provided for ET-1-treated cells (n = 4). For transcripts represented by more than one probeset, the probesets and mean corresponding raw values are listed. RNAs are listed according to translational regulation and in order of functional category then alphabetical order of the gene symbol. AS = Antisense.
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Markou et al. BMC Genomics 2010 11:343 doi:10.1186/1471-2164-11-343