Differential recruitment of cardiomyocyte RNAs to polysomes.Cardiomyocytes were serum-starved (20 h). Polysomes prepared by sucrose density centrifugation. Total and polysomal RNA were extracted and analysed using microarrays. A, A254 profiles for sucrose density centrifugation. B, Heatmap of the mean raw fluorescence values for the 6425 probesets with significantly different expression in polysomal and total RNA pools in unstimulated cells [Log2 scale; 7 (cyan) through 10 (black) to 13 (red)]. C, Functional classification of top 10th percentile transcripts detected in cardiomyocytes. D, Heatmap for the 515 probesets of the top 10th percentile with significantly different expression (FDR < 0.05, >1.5-fold difference) in polysomal and total RNA pools in unstimulated cells [Log2 scale; 9.5 (cyan) through 11.5 (black) to 13.5 (red)].
Markou et al. BMC Genomics 2010 11:343 doi:10.1186/1471-2164-11-343