Open Access Highly Accessed Research article

Powdery mildew fungal effector candidates share N-terminal Y/F/WxC-motif

Dale Godfrey1, Henrik Böhlenius1, Carsten Pedersen1, Ziguo Zhang1, Jeppe Emmersen2 and Hans Thordal-Christensen1*

Author Affiliations

1 Plant and Soil Science Laboratory, Department of Agricultural and Ecology, Faculty of Life Sciences, University of Copenhagen, Denmark

2 Department of Health Science and Technology, Aalborg University, Denmark

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BMC Genomics 2010, 11:317  doi:10.1186/1471-2164-11-317

Published: 20 May 2010

Additional files

Additional file 1:

Table S1. Bgh Y/F/WxC-effector candidates. Data assembly related to the 107 barley powdery mildew fungal Y/F/WxC-proteins.

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Additional file 2:

Figure S1. The Y/F/WxC-motif of the first 35 identified Bgh effector candidates aligns perfectly. Multiple sequence alignment of the derived amino acid sequences of the mature proteins performed by CLUSTALW and visualized using Genedoc. Shading represents conservation of amino acid similarity at each position: residues conserved between sequences are shaded in black.

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Additional file 3:

Figure S2. The Y/F/WxC-motif of the 107 Bgh effector candidates aligns in all but three sequences. Multiple sequence alignment of the derived amino acid sequences of the mature proteins performed by CLUSTALW and visualized using Genedoc. Shading represents conservation of amino acid similarity at each position.

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Additional file 4:

Figure S3. Position of introns in the Bgh Y/F/WxC-effector candidates. The open reading frame is indicated in underlining, Y/F/WxC-coding sequence in bold blue, introns in bold red and untranslated regions in italics. Where sequence has been identified exclusively from genomic sequence, it is indicated in white with black highlight. Where sequence has been identified exclusively from EST sequence, it is indicated in grey highlight.

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Additional file 5:

Table S2. Pgt Y/F/WxC-effector candidates. Data assembly related to the 178 wheat stem rust fungal Y/F/WxC-proteins.

Format: XLS Size: 113KB Download file

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Additional file 6:

Table S3. Pt Y/F/WxC-effector candidates. Data assembly related to the 57 wheat leaf rust fungal Y/F/WxC-proteins.

Format: XLS Size: 48KB Download file

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Additional file 7:

Table S4. Sequences of primers used for semi-quantitative RT-PCR and qPCR.

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