Screening of soldier- or differentiation-specific genes. We performed differential display for the first screening and obtained 40 differentiation-specific and 6 soldier-specific gene candidates. Among these, the expressions of 23 candidates were screened by qRT-PCR (second screening, first qRT-PCR). Thirteen of these were confirmed to be up-regulated during soldier differentiation. Based on Gene Ontology and the BLAST-X algorithm, molecular function terms and biological process terms were assigned to six sequences, which were subsequently quantified by qRT-PCR ("Quantification, 2nd qRT-PCR" in Figure 2).
Ishikawa et al. BMC Genomics 2010 11:314 doi:10.1186/1471-2164-11-314