Impact of chromate challenge on P. putida F1 growth in LB versus M9L media. The optical densities (OD600) of F1 cultures grown aerobically under LB (closed symbols) versus defined minimal M9L media (open symbols) in either the absence or presence of 1 mM K2CrO4 are shown: LB-grown cells with no chromate (closed squares), LB-grown cells with chromate added to 1 mM at the mid-log point (closed circles), M9L-grown cells with no chromate (open squares), and M9L-grown cells with chromate added to 1 mM at the mid-log point (open circles). The point of Cr(VI) addition and cell harvesting for proteomic analyses is presented. Each data point represents the mean of three replicate samples. Error bars indicate one standard deviation.
Thompson et al. BMC Genomics 2010 11:311 doi:10.1186/1471-2164-11-311