Open Access Research article

Proteomics reveals a core molecular response of Pseudomonas putida F1 to acute chromate challenge

Dorothea K Thompson1*, Karuna Chourey2, Gene S Wickham1, Stephanie B Thieman1, Nathan C VerBerkmoes2, Bing Zhang3, Andrea T McCarthy1, Matt A Rudisill1, Manesh Shah4 and Robert L Hettich2*

Author Affiliations

1 Department of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907, USA

2 Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA

3 Department of Biomedical Informatics, Vanderbilt University, Nashville, TN 37232, USA

4 Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA

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BMC Genomics 2010, 11:311  doi:10.1186/1471-2164-11-311

Published: 19 May 2010

Additional files

Additional file 1:

Complete proteome data and dataset comparisons with statistical analysis. Complete dataset detailing the spectral counts for each treatment and its technical replicate. The extended table shows statistically analyzed comparisons between two treatments. Proteins that are highlighted show a two-fold change in expression and 1% False Discovery Rate (FDR).

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Additional file 2:

Proteins detected in all technical replicates for cells grown in LB or M9L media. The table shows proteins detected in all technical replicates of cells grown in LB or M9L media [control and Cr(VI) shock].

Format: XLS Size: 200KB Download file

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Additional file 3:

Proteins unique to LB control compared to M9L control. Table lists proteins unique to unstressed LB grown cells compared to cells grown in M9L medium.

Format: XLS Size: 171KB Download file

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Additional file 4:

Comparison of proteins expressed in unstressed cells grown in LB versus cells grown in M9L medium. Comparison of total proteins expressed in unstressed LB grown cells versus cells grown in M9L medium.

Format: XLS Size: 134KB Download file

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Additional file 5:

Differential protein profile in control and Cr(VI)-shocked M9L-grown cells. Datasheet shows differential protein profile of control and Cr(VI)-shocked cells grown in M9L medium.

Format: XLS Size: 67KB Download file

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Additional file 6:

Unique proteins induced in LB-grown cells and M9L-grown cells in response to chromate. Table lists proteins uniquely expressed under Cr(VI) stress in LB and M9L grown cells.

Format: XLS Size: 20KB Download file

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