Open Access Research article

Temperature increase prevails over acidification in gene expression modulation of amastigote differentiation in Leishmania infantum

Pedro J Alcolea1, Ana Alonso1, Manuel J Gómez2, Alicia Sánchez-Gorostiaga1, Mercedes Moreno-Paz2, Eduardo González-Pastor2, Alfredo Toraño3, Víctor Parro2 and Vicente Larraga1*

Author Affiliations

1 Departamento de Microbiología Molecular y Biología de las Infecciones, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), calle Ramiro de Maeztu, 9, 28040, Madrid, Spain

2 Laboratorio de Ecología Molecular and Unidad de Secuenciación y Bioinformática, Centro de Astrobiología, Instituto Nacional de Técnica Aeroespacial "Esteban Terradas" (INTA) and CSIC, carretera de Ajalvir, Km 4, 28850, Torrejón de Ardoz, Spain

3 Servicio de Inmunología, Centro Nacional de Microbiología, Virología e Inmunología Sanitarias, Instituto de Salud Carlos III (ISCIII), carretera Majadahonda-Pozuelo, Km 2, Majadahonda, Spain

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BMC Genomics 2010, 11:31  doi:10.1186/1471-2164-11-31

Published: 14 January 2010

Additional files

Additional file 1:

Electropherograms of total RNA samples. Figure S1. 18S, 23Sα and 23Sβ spikes, absence of DNA contamination and RNA degradation.

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Additional file 2:

Scatter plots of normalised and contrasted microarray hybridisation data. Figure S2. Spots that fulfill criteria to be considered as differentially regulated are highlighted.

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Additional file 3:

Differentially regulated hypothetical and unknown genes and unresolved clones. Tables S1, S2, S3, S4, S5, S6, S7 and S8. Tables S7 and S8 describe clones containing minicircle sequences.

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Additional file 4:

DAGs (BLAST2GO output). Figures. S3 and S4. GO codes for functions directly annotated on differentially regulated genes found in this study. Each GO code is associated to a custom code to find annotations on genes in Tables 1, 2, 3, 4 and 5.

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Additional file 5:

3'NT/Nase and GT alignments and oligonucleotides for qRT-PCR. Figure S6 (GT and 3'NT/Nase alignments) and Additional file 3: Table S9 (oligonucleotides for qRT-PCR).

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Additional file 6:

Fold change clusters of differentially regulated genes including clone names. Figure S5. Supplementary information for Figure 4 identifying the profiles with the clone numbers.

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