A BAC-based Pax8 transcriptional reporter construct reveals the role of the CNS87 element in the control of Pax8 expression levels. PC Cl3 cells were stable transfected with two reporter constructs derived from a BAC containing the entire Pax8 gene as well as the 5'-flanking region engineered as described in Materials and Methods. Pools of colonies for each construct were analyzed. Protein extracts were prepared and the luciferase activity was measured. Data are expressed as fold change with respect to the transcriptional activity obtained with wtCNS87BAC-LUC, whose value was set at 1.0. Data are means ± SD from 3-4 independent experiments, each performed in duplicate. Statistical analysis uses t test (p < 0.05).
Nitsch et al. BMC Genomics 2010 11:306 doi:10.1186/1471-2164-11-306