Open Access Highly Accessed Research article

Massively parallel pyrosequencing-based transcriptome analyses of small brown planthopper (Laodelphax striatellus), a vector insect transmitting rice stripe virus (RSV)

Fujie Zhang123, Hongyan Guo123, Huajun Zheng4, Tong Zhou5, Yijun Zhou5, Shengyue Wang4, Rongxiang Fang12, Wei Qian12* and Xiaoying Chen12*

Author Affiliations

1 State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

2 National Plant Gene Research Center, Beijing 100101, China

3 Graduate School of Chinese Academy of Sciences, Beijing 100039, China

4 Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, 200032, China

5 Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

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BMC Genomics 2010, 11:303  doi:10.1186/1471-2164-11-303

Published: 13 May 2010

Additional files

Additional file 1:

Distribution of read lengths of viruliferous and naïve Laodelphax striatellus EST libraries. The figure provides statistics of read lengths of L. striatellus ESTs sequencing by the Roche 454-FLX platform.

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Additional file 2:

List of Wolbachia genes identified in L. striatellus EST libraries. This table provides a list of genes from endosymbiotic bacteria Wolbachia that are expressed in cells of L. striatellus.

Format: HTM Size: 73KB Download file

Open Data

Additional file 3:

List of possible fungal genes identified in L. striatellus EST libraries. The table provides a list of genes that possibly expressed by endosymbiotic fungi, including yeast-like symbiotes.

Format: HTM Size: 1.6MB Download file

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Additional file 4:

L. striatellus genes abundantly transcribed in the viruliferous sample. The table provides a list of top 30 genes that highly expressed in the sample of viruliferous insects.

Format: HTM Size: 45KB Download file

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Additional file 5:

List of genes selected for RT-PCR assay. This table shows the general feature of genes selected for RT-PCR analysis.

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Additional file 6:

Sequences of primers used in RT-PCR profiling.

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