Table 2

MT-PCR-HRM pooling strategy to accommodate for variable genome size.

Organism

1C genome (Mb)

No 384 well plates

No super pools

No matrix pools

No single pools

Total required simplex MT-PCR-HRM

Total required triplex MT-PCR-HRM


Brachypodium distachyon

300

24

1

10

54*

64

22


Potato

850

66

3

30

54

87

29


Barley

5500

430

17

30

54

101

35


Wheat

16000

1250

50

30

54

134

45


Swine

2600

203

8

30

54

92

31


Cattle

3100

242

10

30

54

94

32


Estimated number of pools and MT-PCR-HRM reactions required to find a single marker (or 3 markers simultaneously) within the denoted genomes, assuming a 3× coverage and 100 Kb average insert size for the corresponding BAC libraries. Note: *1× coverage = 18 single pools (see Figure 1); 3× coverage = 54 single pools

Vu et al. BMC Genomics 2010 11:301   doi:10.1186/1471-2164-11-301

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