Figure 2.

The need for pre-amplification: MT-PCR-HRM using freezing stocks of a super pool (25 × 384 well plates). A first order differential plot showing failed amplification by direct PCR (35 cycles, red line) and in the negative water control (blue line) but a strong signal after preamplification applying MT-PCR-HRM (green line) for STS marker M45.

Vu et al. BMC Genomics 2010 11:301   doi:10.1186/1471-2164-11-301
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