Expression levels of some of the known miRNAs determined by RNase protection assay. The relative expression levels of some of the differentially regulated miRNAs were determined using RPA. Briefly, total RNA from indicated cells was incubated with a labelled probe specific for a given miRNA and eventually treated with ribonuclease as described in the "Methods". The protected fragments, suggesting presence of specific transcripts, were first separated on 12% urea PAGE and then visualized by phosphorimager. Loading control was transcripts corresponding to RNU6B visualized using RPA.
Vaz et al. BMC Genomics 2010 11:288 doi:10.1186/1471-2164-11-288