Predicted novel miRNAs. A. A partial list of novel miRNAs predicted from deep sequencing data is displayed along with chromosomal location and the scores from different prediction tools. B. The precursor sequence and the secondary structure of the novel miRNAs. The highlighted regions in blue and yellow colour indicate the presence of 5p and 3p mature miRNA sequences, respectively. Note that the sequenced mature putative miRNAs map to the stem part of the structure. C. The expressions of these miRNAs were independently validated by RPA. RPA was carried out as described in the legend for Figure 7 using total RNA from normal PBMC and K562 cell lines. The phosphor imager images are shown. RNU6B transcripts were used as a control. Some of the miRNA star sequences were also detected. The brightness/contrast have been changed to normalize the signals across different probes.
Vaz et al. BMC Genomics 2010 11:288 doi:10.1186/1471-2164-11-288