Open Access Research article

Differences in transcription between free-living and CO2-activated third-stage larvae of Haemonchus contortus

Cinzia Cantacessi1, Bronwyn E Campbell1, Neil D Young1, Aaron R Jex1, Ross S Hall1, Paul JA Presidente2, Jodi L Zawadzki2, Weiwei Zhong3, Boanerges Aleman-Meza4, Alex Loukas5, Paul W Sternberg6 and Robin B Gasser1*

Author Affiliations

1 Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia

2 Department of Primary Industries, Attwood, Victoria, Australia

3 Department of Biochemistry and Cell Biology, Rice University, Houston, Texas, USA

4 Department of Information Technology, Polytechnic University of Victoria, Mexico

5 James Cook University, Cairns, Queensland, Australia

6 Biology Division, California Institute of Technology, Pasadena, California, USA

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BMC Genomics 2010, 11:266  doi:10.1186/1471-2164-11-266

Published: 27 April 2010

Additional files

Additional file 1:

Protein motifs. InterPro domains predicted peptide sequences encoded in either the ensheathed (= L3) or exsheathed (= xL3) third larval stage of Haemonchus contortus.

Format: XLS Size: 235KB Download file

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Additional file 2:

Gene ontology (GO). Terms (i.e. 'biological process', 'cellular component' and/or 'molecular function') representing proteins inferred to be encoded by either the ensheathed (= L3) or exsheathed (= xL3) third larval stage of Haemonchus contortus.

Format: XLS Size: 134KB Download file

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Open Data

Additional file 3:

Prediction of biological pathways. Pathways predicted for molecules inferred to be encoded in either the ensheathed (= L3) or exsheathed (= xL3) third larval stage of Haemonchus contortus.

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Additional file 4:

List of Caenorhabditis elegans orthologues. C. elegans orthologues of expressed sequence tags (ESTs) and genome survey sequences (GSS) representing either the ensheathed (= L3) or exsheathed (= xL3) third larval stage of Haemonchus contortus.

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Additional file 5:

Expressed sequence tags (ESTs) unique to the ensheathed third larval stage (L3). Bioinformatic characterisation of ESTs encoding molecules uniquely transcribed in the L3 of Haemonchus contortus with orthologues in Caenorhabditis elegans and other parasitic nematodes.

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Additional file 6:

Expressed sequence tags (ESTs) unique to the exsheathed third larval stage (xL3). Bioinformatic characterisation of ESTs encoding molecules uniquely transcribed in the xL3 of Haemonchus contortus with orthologues in Caenorhabditis elegans and other parasitic nematodes.

Format: DOC Size: 71KB Download file

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Additional file 7:

Probabilistic genetic interaction network predictions. A list of Caenorhabditis elegans orthologues of expressed sequence tags (ESTs) unique to either the ensheathed (= L3) or exsheathed (= xL3) third larval stage of Haemonchus contortus for which probabilistic genetic interaction networks were predicted. Interacting genes are listed according to decreased cut-off scores (see Methods).

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Additional file 8:

Reverse-transcription polymerase chain reaction (rtPCR). The sequences of oligonucleotide primers used in rtPCR (see Methods).

Format: DOC Size: 143KB Download file

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