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Open Access Research article

Global transcriptome analysis of spore formation in Myxococcus xanthus reveals a locus necessary for cell differentiation

Frank-Dietrich Müller14, Anke Treuner-Lange25, Johann Heider3, Stuart M Huntley1 and Penelope I Higgs1*

Author Affiliations

1 Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, 35043, Marburg, Germany

2 Institute for Microbiology and Molecular Biology, University of Giessen, 35392 Giessen, Germany

3 Laboratory for Microbiology, Department of Biology, Philipps University Marburg, 35043, Marburg, Germany

4 Current address: Department of Microbiology, Ludwig Maximilians University Munich, 82152, Planegg-Martinsried, Germany

5 Current address: Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, 35043, Marburg, Germany

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BMC Genomics 2010, 11:264  doi:10.1186/1471-2164-11-264

Published: 26 April 2010

Additional files

Additional file 1:

Confirmation of microarray results by quantitative real-time PCR. Quantitative real-time PCR analysis (black bars) of select genes designated as significantly up- (sigB, sigC, mspC, prU) or down-regulated (Mxan_5543, atpE), or not significantly regulated (devR) in the microarray data (white bars). The data are shown as levels of each transcript at the indicated times relative to the respective level in uninduced vegetative cells. cDNA was generated from equal amounts of the RNA templates used for the microarray analysis and amplified using gene-specific primers (see Materials and Methods for details).

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Additional file 2:

M. xanthus orf functional category assignment. The list of M. xanthus protein coding genes assigned by JCVI and the associated main role/subrole assignments used in this study. Regulation patterns assigned in this study are also included. The tally of functional categories with respect to significantly regulated genes is included. Genes associated with developmental phenotypes are listed.

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Additional file 3:

Strains and plasmids used in this study. The list of strains and plasmids and associated genotypes used in this study

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Additional file 4:

Primers used for real-time PCR analysis. The list of primers and associated sequences used for real-time PCR analysis (Additional File 1) and reverse transcriptase PCR analysis (Figure 5) in this study.

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