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Open Access Highly Accessed Research article

De novo sequencing and analysis of the American ginseng root transcriptome using a GS FLX Titanium platform to discover putative genes involved in ginsenoside biosynthesis

Chao Sun1, Ying Li1, Qiong Wu1, Hongmei Luo1, Yongzhen Sun1, Jingyuan Song1, Edmund MK Lui2 and Shilin Chen1*

Author Affiliations

1 Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences, No.151, Malianwa North Road, Haidian District, Beijing 100193, China

2 Department of Physiology and Pharmacology, The University of Western Ontario, Dental Sciences Building, London, ON N6A 5C1, Canada

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BMC Genomics 2010, 11:262  doi:10.1186/1471-2164-11-262

Published: 24 April 2010

Additional files

Additional file 1:

Summary of the annotation sources for American ginseng.

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Additional file 2:

Most abundant transcripts in the root of American ginseng.

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Additional file 3:

Pathway assignment based on KEGG. A) Classification based on metabolism categories; B) Classification based on secondary metabolism categories.

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Additional file 4:

Enzymes involved in the biosynthesis of steroids based on KEGG. Enzymes that were found in this study are marked with red rectangles.

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Additional file 5:

Putative CYP450 and UGT genes in the 454 cDNA library.

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Additional file 6:

Primers used in this study.

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