Figure 2.

Edited miRNAs. A) miRNAs of which at least 10% was found to be edited. Shown are the precursors in their predicted folding structure. Red sequences represent miRNAs that are covered in our DGE profiles, bold "a"s along numbers indicate known and newly detected editing positions. B) Fractions of edited vs consensus miRNAs. Columns represent duplicates from each tissue, rows indicate miRNAs and edited position on the precursor. The red intensity reflects the fraction of edited miRNAs (edited miRNAs on this position/(edited + consensus miRNAs)). The triplets on the right side reflect the wild-type allele of which the middle adenosine is edited. C) qPCR data for mir-377. The first two bars describe results obtained from brain, the only tissue from which mir-377 was cloned. The ΔCt in brain corresponds to a ~2.7 fold higher expression of the consensus compared to the edited miRNA. Testis served as a negative input control. The specificity of the primers is indicated by the synthetic consensus and edited miRNA, mixed in a 1:4 ratio (cs: consensus, ed: edited). nc: primers only.