Table 1

Summary of sequence reads, coverage, and quality score.

Strain

Genomic DNA

Total reads

Mapped reads

Coverage

Quality score 40

(ng/μl)

(million)

(%)

(fold)

(%)


BEST 195

207

15.3

79.1

131

86.3

Marburg 168

140

13.2

92.2

111

99.7


Genomic DNA was extracted from B. subtilis natto BEST195 isolated from Miyagino-based natto, and B. subtilis Marburg 168 (1A1). The short paired-end reads were generated by the Illumina genome analyzer GA2. The generated paired-end reads were mapped to the Marburg 168 reference genome using MAQ software. At each base, a quality score was statistically calculated based on the reads using MAQ. The quality scores describe the confidence that the base is correctly called.

Nishito et al. BMC Genomics 2010 11:243   doi:10.1186/1471-2164-11-243

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