Concatenating sorted scaffolds to constitute scaffolds. The de novo assembled scaffolds were combined with the reference-guided draft to fill the gaps among the scaffolds. (Left) Two adjacent scaffolds that overlapped were merged into one larger scaffold. (Right) If a subsequence was inserted in the reference-guided draft between two adjacent scaffolds, such scaffolds and the inserted subsequence were concatenated into one scaffold.
Nishito et al. BMC Genomics 2010 11:243 doi:10.1186/1471-2164-11-243