Table 1

Correlation of transcripts and proteins of the polyamine biosynthetic pathway following PfSpdSyn inhibition.

Fold change (relative to control)


Annotation

PlasmoDB ID

Hours post invasion

Oligos representing gene

Oligonucleotide array

RT-qPCR

Proteomics


18

1

-2.304

-3.472

nd


Adenosine deaminase, putative

PF10_0289

25

1

-2.907

-5.495

nd


30

1

-2.778

-3.413

-3.030


18

1

-3.559

-4.651

nd


Purine nucleoside phosphorylase/uridine phosphorylase, putative

PFE0660c

25

1

-3.731

-5.882

-2.222


30

1

-3.367

-4.348

nd


18

1

-1.961

-3.115

nd


Phosphoethanolamine N-methyltransferase, putative

MAL13P1.214

25

1

-3.401

-5.495

nd


30

1

-3.774

-4.049

nd


18

2

-1.157

1.123

-1.185

nd


Spermidine synthase

PF11_0301

25

2

-1.038

1.300

-1.289

nd


30

2

-1.035

1.320

+1.087

nd


18

1

+1.324

-1.362

nd


Lysine decarboxylase, putative

PFD0285c

25

1

+2.426

+1.106

nd


30

1

+1.954

+1.591

nd


18

1

-1.300

nd

-3.030


Ornithine aminotransferase, PFF0435w

25

1

+1.044

nd

nd


30

1

+1.182

nd

nd


18

1

-1.597

nd

-2.857


S-adenosyl methionine synthethase,

PFI1090

25

1

-1.631

nd

-2.222


30

1

-1.761

nd

nd


18

1

1.347

nd



Eukaryotic translation initiation factor 3 subunit 10, putative

(endogenous control)

PFL0625c

25

1

1.210

endogenous control

nd



30

1

1.221

nd


The array and quantitative real-time PCR (RT-qPCR) datasets showed a high degree of concordance revealed by a Pearson correlation (r) of 0.88. Fold changes for transcripts and proteins are given at each time point. Differentially affected genes and their cognate proteins generally exhibited the same direction and level of regulation.

Becker et al. BMC Genomics 2010 11:235   doi:10.1186/1471-2164-11-235

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