Osteogenic Differentiation. MSC, passage 11 from each age were grown to confluence and exposed to osteogenic induction medium for 14 days and stained with Alizarin Red. Cells from each age group were positive after 14 days (A-C; 2mo, 8mo, 26mo) whereas no staining was seen in cells treated with control medium (D,E, F; 2mo, 8mo, 26mo).
Wilson et al. BMC Genomics 2010 11:229 doi:10.1186/1471-2164-11-229