ELISA measurement of VEGF secretion. ELISA was performed as described in Methods. MSC from each age were grown to confluence in parallel and VEGF measured in the medium 24h after replacement. Significantly less VEGF was secreted from cells taken from mice at age 26 mo (n=4; p<0.02).
Wilson et al. BMC Genomics 2010 11:229 doi:10.1186/1471-2164-11-229