Table 1

Summary of genotyping results.

Locus

Locus reference

Number of individuals genotyped in study population

Predicted allele size

Observed allele size range

Number of alleles obs.

He

Ho

Est. null allele freq. (CERVUS v3.0)

Mean estimated error rate (CERVUS v3.0)

Multiplex set


ZF01-020

This study

320

178

160-261

15

0.69

0.59

0.075

0.007

3

ZF01-025

This study

334

211

188-217

11

0.78

0.77

0.005

0

2, 3

TG01-040

This study

307

286

286-294

7

0.69

0.70

-0.011

0

2

ZF01-054a

This study

308

185

157-188

13

0.89

0.75

0.083

0.002

2

ZF01-139b

This study

330

188

156-209

4

0.53

0.55

-0.007

0.040

4

ZF01-136

This study

299

367

333-373

10

0.73

0.55

0.138$

0

2

ZF01-170c

[47]

21

316

257-305

9

0.83

0.86

-0.032

-

4

ZF01-161

This study

333

159

125-168

8

0.70

0.74

-0.036

0

4

ZF01-190

This study

329

342

308-366

12

0.83

0.87

-0.026

0

3

ZF01-196

This study

334

292

270-304

7

0.74

0.77

-0.014

0

2, 3, 4

Tgu12

This study

331

282

248-273

10

0.79

0.75

0.026

0.016

3

ZF01-180b

[43]

331

213

166-218

8

0.76

0.66

0.071

0.060

4

ZF01-081b

This study

337

233

130-154

5

0.55

0.51

0.031

0.077

4

ZF02-068

This study

292

185

121-152

7

0.70

0.50

0.157$

0.010

2

ZF02-038b

This study

301

233

211-245

11

0.77

0.66

0.074

0.085

2

TG02-078

[43]

314

309

308-321

6

0.73

0.76

-0.024

0

1

TG02-088

[43]

310

268

263-269

5

0.73

0.77

-0.034

0

1

Ase44

[64]

303

268

308-329

8

0.81

0.79

0.010

0.006

2

ZF02-128

This study

301

374

365-435

9

0.83

0.79

0.022

0.005

2

ZF02-129

This study

329

169

124-172

11

0.83

0.82

0.004

0.005

3

ZEST09-005

Dawson et al. unpublished

316

168

155-165

5

0.74

0.67

0.047

0

1

Smm4

[65]

313

332

332-341

6

0.57

0.50

0.049$

0.018

1

Cpi7

[66]

308

128

119-131

5

0.54

0.55

-0.016

0

1

ZEST09-018

Dawson et al. unpublished

289

285

282-293

8

0.70

0.75

-0.039

0

1

ZEST09-021

Dawson et al. unpublished

311

119

114-121

5

0.47

0.41

0.096

0

1

ZEST09-025

Dawson et al. unpublished

281

167

164-170

6

0.71

0.69

0.014

0

1


Information is for the 26 zebra finch (Taeniopygia guttata) microsatellite markers used to create the linkage maps for the zebra finch chromosomes 1, 1A, 2 and 9. See additional file 1: Table S1 for more information.

He, Ho expected and observed heterozygosity (calculated using CERVUS v3.0)

$, Markers with null alleles; null alleles were detectable by following their segregation through the pedigree. Null alleles were rescored as allele 99 before running through CriMap. Null allele frequencies are calculated using the original genotypes and are based on the excess of homozygous individuals.

a, Excluded after CHROMPIC revealed an excess of double recombination events with adjacent markers, indicative of high error rate.

b, Excluded from linkage maps as parent-offspring mismatches estimated the error rate > 0.02

c, Could not be accurately scored after multiplexing.

Ball et al. BMC Genomics 2010 11:218   doi:10.1186/1471-2164-11-218

Open Data