Table 2 |
|||||
|
Quantitative (Real-Time) RT-PCR information |
|||||
|
Feature |
Tentative I.D. |
Primer pair (Forward/Reverse) |
Amplicon Length (bp) |
Tm (°C) |
Amplification Efficiency (%) |
|
|
|||||
|
Contig1127 |
P450 (CYP6) |
ACTTCCCGCTGGATACAGACAT/GGATAAGACATCGTCTGGATTGTTG |
101 |
76 |
90.91 |
|
Contig1103 |
P450 (CYP6) |
AATGACTGCTTCGGTGCTGAA/ATTCCATGTCCCTACGATTGTGT |
113 |
79 |
90.80 |
|
DPG001G12 |
P450 (CYP4) |
CCGTAATCCCACAATGTTCGA/CCAAGGCAAAGTCTAGGTCCAT |
117 |
78 |
92.41 |
|
Contig64 |
P450 (CYP6) |
GCAAGAGGAATCAACCGCTAA/CTATGCTGCCTCAGCTCGTTATT |
128 |
79 |
86.46 |
|
Contig608 |
P450 (CYP6CR1) |
GAGGAACCACATAGTTGTCATGGA/CAAAAGGGAGGCGGATGTTA |
141 |
73 |
93.3 |
|
Contig160 |
P450 (CYP6) |
AATGATTGCTTCGGCACTGAA/GGATTTGAGTAATTCCATGCTCCTA |
113 |
76 |
105.64 |
|
MPB029F09 |
P450 (CYP6) |
ACTGGTAACGGACTACGATCACTTT/TGAACGCAATACTTTCCATTCG |
115 |
75 |
89.16 |
|
MPB019E07 |
HMGR |
CCAATCACCCGTGGGAAGT/CGAAGTGGAGGTTGCTGTTCA |
82 |
81 |
95.45 |
|
Contig126 |
GGPPS |
TGAACGTGCCCAAAGAGAATT/TCGGCTAGTTTAGCTCGGATATTT |
101 |
77 |
92.12 |
|
|
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|
Aw et al. BMC Genomics 2010 11:215 doi:10.1186/1471-2164-11-215 |
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