Gene models (A, C) and protein domain organization (B, D) of the seven human ZNF genes at 8q24.3 (A, B) and their murine orthologs (C, D). In the gene models (A, C) exons are shown as boxes (white parts are untranslated, filled parts are translated), introns as solid lines. KRAB-A, KRAB-B and non-KRAB domain encoding sequences are shaded in dark blue, light blue and black, respectively. Numbers above exons/introns indicate their size (bp). Genes are drawn to scale with smaller exons additionally magnified for more detail. Introns interrupted by two perpendicular bars are not fully drawn out. Names of genes and proteins are given on the left of each model. Mouse and rat orthologs are indicated by prefixes mm (Mus musculus) or rn (Rattus norvegicus). * in the ZNF252 5-exon model indicates out of frame KRAB-A coding sequence, ** designates a degenerate KRAB-B box disrupted by a stop codon. The genes' direction of transcription is given by "+" or "-" (towards telomere or centromere, respectively). Genomic order of the human genes is indicated by the arrow in A. Exact genomic locations are displayed for mouse and rat genes (based on UCSC assemblies mm9 and rn4). Small arrows denote positions of presumable translational starts. Drawn to scale protein architectures (B, D) depict KRAB-A (dark blue), KRAB-B (light blue) and C2H2 ZNF domains (numbered boxes; black boxes represent complete, grey boxes degenerate or non-canonical fingers). Only fingers with at least two of the four conserved C2H2 residues were considered.
Lorenz et al. BMC Genomics 2010 11:206 doi:10.1186/1471-2164-11-206