Genomic organization of the human ZNF cluster at chromosomal region 8q24.3 and its syntenic regions in mouse and rat. A. FISH of ZNF gene PAC clones on combed DNA. Orientation is given by indicating the centromeric (centr) and telomeric (tel) directions. Numbers on the depicted DNA indicate sizes within the piece of combed DNA. B. Map of the sequenced individual PAC clones at the 8q24.3-8qter locus (open bars) and their alignment/assembly within the whole contig (genomic assemblies with respective GenBank accessions shown as grey bars). Range, size and chromosomal position of the whole contig is reflected in the depicted chromosomal region on top. The ZNF gene models (see Table 1, Figure 2 and text) are indicated as colored boxes encompassing the sequence stretch from first to last exon. Genomic regions syntenic to human 8q24.3 in mouse and rat are depicted at the bottom. Same coloring designates orthologs. The three human genes depicted in black lack orthologs in mouse and rat (exception: presumable KRAB-B remnant of ZNF252 ortholog in rat, see text). Isolated, often degenerate sequences for KRAB-A ("pin" with square head), KRAB-B ("pin" with round head) and C2H2 zinc fingers (open rectangle with label "pseudo") are displayed at their respective position. Two other well characterized genes (RPL8, COMMD5) in the region are shown by striped boxes. The coding strand of each element is indicated by positioning above or below the depicted chromosomal region. Based on genome assemblies UCSC hg18 (human), mm9 (mouse) and rn4 (rat).
Lorenz et al. BMC Genomics 2010 11:206 doi:10.1186/1471-2164-11-206