Additional file 3.
Quantitative PCR validation of microarray data for select human and mouse genes. Twelve human genes were subject to qPCR validation in the human Coriell-18 and ECACC-6 sets. Ten mouse genes were subject to qPCR validation in the murine Nf1-Mouse-12 set. Both microarray and qPCR expression values in NF1-affecteds and Nf1+/- mice were normalized to expression values in NF1-unaffecteds or wild-type mice, respectively. Expression values in NF1-unaffecteds and wild-type mice were arbitrary set at 1.0. Red bars denote mean expression in NF1-affecteds on microarrays, blue bars denote mean expression in NF1-unaffecteds on microarrays; orange bars denote mean expression in NF1-affecteds by qPCR; and green bars denote mean expression in NF1-unaffecteds by qPCR. Gene names are shown below each set of bars. Sample set names are shown on top of each plot. Error bars are equal to one standard deviation. Asterisks above bars denote genes validated by qPCR (nominal P value < 0.05).
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Pemov et al. BMC Genomics 2010 11:194 doi:10.1186/1471-2164-11-194