Table 2

Genes that are involved in major glutamate-related pathways and that are differentially expressed between the glu1-2 mutant and the wild-type mutant

EC #

Enzyme/protein

Abbreviation

Gene ID

log2 root

log2 leaf


1.4.1.7

Fd-dependent glutamate synthase

Fd-GOGAT 1/GLU1

AT5G04140

-5.738


1.4.1.7

Fd-dependent glutamate synthase

Fd-GOGAT 2/GLU2

AT2G41220

1.070


1.4.1.14

NADH-dependent glutamate synthase

NADH-GOGAT

AT5G53460

0.712


1.7.1.1

nitrate reductase

NIA2/NR2

AT1G37130

0.438


1.7.7.1

nitrite reductase

NIR1

AT2G15620

0.687


6.3.1.2

glutamine synthetase

GLN1.1

AT5G37600

1.296


6.3.1.2

glutamine synthetase

GLN1.3

AT3G17820

0.669


6.3.1.2

glutamine synthetase

GLN1.4

AT5G16570

-0.580


6.3.5.4

asparagine synthetase

ASN1

AT3G47340

-2.913


6.3.5.4

asparagine synthetase

ASN2

AT5G65010

-0.486


3.5.1.1

asparaginase

AT3G16150

-0.481


2.6.1.1

aspartate aminotransferase

ASP1

AT2G30970

0.582


2.6.1.1

aspartate aminotransferase

ASP2

AT5G19550

1.132


2.6.1.1

aspartate aminotransferase

ASP3

AT5G11520

0.709


2.6.1.1

aspartate aminotransferase

ASP4

AT1G62800

0.354


2.6.1.2

alanine:2-oxoglutarate aminotransferase

AlaAT1

AlaAT2

AT1G17290

AT1G72330

0.438


2.6.1.2/2.6.1.4

glutamate:glyoxylate aminotransferase

GGAT2

AT1G70580

-0.680


glycine decarboxylase complex -- H protein

AtGDH3

AT1G32470

0.593


glycine decarboxylase complex -- H protein

AtGDH1

AT2G35370

0.768


1.8.1.4

glycine decarboxylase complex -- L protein

AtmLPD1

AT3G17240

0.689


1.4.4.2

glycine decarboxylase complex -- P protein

AtGLDP1

AT4G33010

0.439

-1.126


1.4.4.2

glycine decarboxylase complex -- P protein

AtGLDP2

AT2G26080

-0.532


2.1.2.10

glycine decarboxylase complex -- T protein

AtGDT1

AT1G11860

-0.600


2.1.2.1

serine hydroxymethyltransferase

SHM1

AT4G37930

-0.513


2.1.2.1

serine hydroxymethyltransferase

SHM3

AT4G32520

0.532


2.1.2.1

serine hydroxymethyltransferase

SHM4

SHM5

AT4G13930

AT4G13890

0.535


4.1.1.15

glutamate decarboxylase

GAD3

GAD4

AT2G02000

AT2G02010

1.441


2.6.1.19

γ-aminobutyric acid transaminase

GABA-T1

AT3G22200

0.720


1.2.1.24

succinic semialdehyde dehydrogensase

SSADH1

AT1G79440

0.516


1.1.1.86

ketolacid reductoisomerase

KARI

At3g58610

0.380


4.2.1.9

dehydroxyacid dehydratase

DHAD

At3g23940

0.857


6.3.2.2

glutamate-cysteine ligase

GSH1

AT4G23100

0.587


6.3.2.3

glutathione synthetase

GSH2

AT5G27380

0.855


1.4.1.3

glutamate dehydrogenase

GDH2

AT5G07440

1.126


2.3.1.1

N-acetyltransferase

NAGS

AT4G37670

0.755


6.3.5.5

carbamoyl-phosphate synthase

CPS

AT3G27740

0.696


6.3.5.5

carbamoyl-phosphate synthase

CPS

AT1G29900

0.701


2.1.3.3

ornithine carbamoyltransferase

OTC

AT1G75330

0.436


6.3.4.5

arginosuccinate synthase

AS

AT4G24830

0.596


nitrate transporter

NRT1.1

AT1G12110

0.432


nitrate transporter

NRT1.3/NTP3

AT3G21670

-0.703


nitrate transporter

NRT1.5

AT1G32450

0.382


nitrate transporter

NRT1.7

AT1G69870

0.501

1.760


nitrate transporter

AtNRT2.7

AT5G14570

0.546


nitrate uptake

NRT3.1/NAR2.1

AT5G50200

0.939


nitrate/proton antiporter

AtCLCa

AT5G40890

-0.689


ammonium transporter

AMT2.1

AT2G38290

1.495


vacuolar tonoplast intrinsic protein

AtTIP2;1

AT3G16240

-0.554


vacuolar tonoplast intrinsic protein

AtTIP2;3

AT5G47450

-1.105


amino acid permease

AAP1

AT1G58360

-0.523


amino acid permease

AAP4

AT5G63850

-0.586

-0.470


amino acid permease

AAP5

AT1G44100

0.901


amino acid permease

AAP6

AT5G49630

-0.532


glutamate influx

LHT1

AT5G40780

-0.411


glutamate receptor family protein

AtGLR1.4

AT3G07520

-0.453


glutamate receptor family protein

AtGLR3.2

AT4G35290

-0.613


glutamate receptor family protein

AtGLR3.7

AT2G32400

0.338


List of genes encoding enzymes involved in N-assimilation and glutamate biosynthesis and transformation described in the text, and whose expression is affected by knocking down Fd-GOGAT1 in the glu1-2 mutant. Most of the listed enzymes are also depicted in Figure 8. The presence of two gene IDs in some boxes indicates a nondiscriminating probe, i.e. a probe recognising more than one transcript so that a change in expression can not be attributed to a specific gene.

Kissen et al. BMC Genomics 2010 11:190   doi:10.1186/1471-2164-11-190

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