Additional file 2.
Cloning strategy and results. (a) Target LRR-RLK sequences without stop codons are RT-PCR amplified, agarose gel purified and recombined with the pDONR/ZeoR vector by BP clonase to create pENTR-LRR-RLK entry clones. Final expression constructs are created by performing LR clonase-mediated DNA recombination between the pENTR-LRR-RLK clones and the destination vectors that contain GFP or FLAG epitope tags. (a) The cloning results of the predicted LRR-RLKs in Arabidopsis.
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Gou et al. BMC Genomics 2010 11:19 doi:10.1186/1471-2164-11-19