Gene transformation constructs generated in this study. Four GatewayR-compatible cloning vectors developed specifically in this study. All the four vectors were derived from pBIB vectors  by inserting the GatewayR module and BASTA resistance gene. GatewayR-mediated addition of GFP and FLAG epitope tags to the C-terminal ends of target sequences in vectors pB35GWG and pB35GWF. The attB sites are from the recombination between attL and attR sites. The target LRR-RLK sequence without stop codon is inserted between the attB1 and attB2 sites. To make the sequence in-frame with the epitope tags, one extra G is attached to the end of the C-terminus of the target sequence. Amino acids are indicated with a single-letter code. Additional amino acids from attB sites and linking sequences in destination vectors are added to the final protein.
Gou et al. BMC Genomics 2010 11:19 doi:10.1186/1471-2164-11-19