Systematic analysis of off-target effects in an RNAi screen reveals microRNAs affecting sensitivity to TRAIL-induced apoptosis
1 Work performed at: Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
2 Department of Systems Biology, Harvard Medical School, Boston, USA
3 European Bioinformatics Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
4 Terrence Donnelly Centre for Cellular and Biomolecular Research, 160 College Street, Toronto, Ontario, Canada
BMC Genomics 2010, 11:175 doi:10.1186/1471-2164-11-175Published: 15 March 2010
Additional file 1:
Complete screen results (screen_results.zip). Complete results from the siRNA, presented as a mini-website as produced by the cellHTS software
Additional file 2:
Supplementary figures (supplementary_figures.pdf). Figures S1 and S2 containing detailed results from confirmation experiments for all 16 genes targeted by siRNAs from the screen which reproducibly reduce sensitivity to TRAIL-induced cell death.
Format: PDF Size: 26KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 3:
Ranking of siRNAs after removal of siRNAs with enriched seed sequences (no_enriched_seeds.csv). Ranking of siRNAs used in the screen according to their score, after any siRNA containing an enriched seed had been removed and data reanalysed.
Format: CSV Size: 1.3MB Download file
Additional file 4:
Sequences of siRNAs used (siRNAs.csv). List of sequences, suppliers and catalogue numbers for non-library siRNAs.
Format: CSV Size: 4KB Download file
Additional file 5:
Sequences of qRT-PCR primers (primers.csv). List of primer sequences used along with product lengths and amplification efficiency for each pair.
Format: CSV Size: 2KB Download file