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Open Access Highly Accessed Research article

Systematic analysis of off-target effects in an RNAi screen reveals microRNAs affecting sensitivity to TRAIL-induced apoptosis

Ian Sudbery12, Anton J Enright13, Andrew G Fraser14 and Ian Dunham13*

Author Affiliations

1 Work performed at: Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, UK

2 Department of Systems Biology, Harvard Medical School, Boston, USA

3 European Bioinformatics Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, UK

4 Terrence Donnelly Centre for Cellular and Biomolecular Research, 160 College Street, Toronto, Ontario, Canada

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BMC Genomics 2010, 11:175  doi:10.1186/1471-2164-11-175

Published: 15 March 2010

Additional files

Additional file 1:

Complete screen results (screen_results.zip). Complete results from the siRNA, presented as a mini-website as produced by the cellHTS software

Format: ZIP Size: 3.1MB Download file or display content in a new window

Open Data

Additional file 2:

Supplementary figures (supplementary_figures.pdf). Figures S1 and S2 containing detailed results from confirmation experiments for all 16 genes targeted by siRNAs from the screen which reproducibly reduce sensitivity to TRAIL-induced cell death.

Format: PDF Size: 26KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

Ranking of siRNAs after removal of siRNAs with enriched seed sequences (no_enriched_seeds.csv). Ranking of siRNAs used in the screen according to their score, after any siRNA containing an enriched seed had been removed and data reanalysed.

Format: CSV Size: 1.3MB Download file

Open Data

Additional file 4:

Sequences of siRNAs used (siRNAs.csv). List of sequences, suppliers and catalogue numbers for non-library siRNAs.

Format: CSV Size: 4KB Download file

Open Data

Additional file 5:

Sequences of qRT-PCR primers (primers.csv). List of primer sequences used along with product lengths and amplification efficiency for each pair.

Format: CSV Size: 2KB Download file

Open Data