Table 4

The envelope proteome of H. mustelae determined by LC-MS

Ranka

Locus

Annotation

Mw Da

MOWSE Scoreb

Coveragec%

emPAIed

Mol%


1

HMU14370

cft ferritin

19,056

455

53

2.72

5.74

2

HMU03320

ahpC alkyl hydroperoxide reductase

22,088

806

68

2.48

5.22

3

HMU12840

aroQ 3-dehydroquinate dehydratase

17,477

105

21

2.16

4.55

4

HMU14250

undefined product; COG4969, tfp pilus assembly protein, major pilin PilA [cell motility and secretion/Intracellular trafficking and secretion]

15,880

241

50

1.42

3

5

HMU00320

undefined product; no putative conserved domains detected, hypothetical protein Lreu23DRAFT_1924 [L. reuteri 100-23]: 35% ID

8,942

158

33

1.37

2.89

6

HMU14210

fldA flavodoxin 1

18,147

231

25

1.31

2.76

7

HMU03050

ureA fusion of urease beta and gamma subunits

25,199

683

60

1.15

2.43

8

HMU03060

ureB urease alpha subunit

61,146

1,218

37

1.048

2.21

9

HMU04000

groEL 60 kD chaperonin (cpn60)

57,443

1,714

51

1.035

2.18

10

HMU12690

possible bacterioferritin

17,198

380

32

1.015

2.14

11

HMU03500

putative amino-acid transporter periplasmic solute-binding protein

30,533

626

52

0.96

2.02

12

HMU01210

tpx probable thiol peroxidase

17,993

350

38

0.83

1.75

13

HMU00630

putative autotransporter protein

151,727

1,371

25

0.82

1.73

14

HMU13930

putative exported protein; COG1464, NlpA, ABC-type metal ion transport system, periplasmic component/surface antigen [inorganic ion transport and metabolism]

28,862

425

30

0.82

1.73

15

HMU01180

putative membrane-anchored cell surface protein

281,808

2,432

22

0.81

1.72

16

HMU03120

tuf elongation factor TU

43,570

850

48

0.77

1.64

17

HMU04030

petA putative putative ubiquinol-cytochrome C reductase iron-sulfur subunit

17,916

227

46

0.77

1.64

18

HMU06410

undefined product; No putative conserved domains detected, hypothetical protein Hac_1480 [Helicobacter acinonychis str. Sheeba]: 29% ID

20,229

254

18

0.77

1.64

19

HMU03990

groES 10 kD chaperonin (cpn10)

10,185

157

31

0.71

1.51

20

HMU09770

trxA thioredoxin

11,553

194

28

0.71

1.51

21

HMU08630

putative outer membrane autotransporter

155,228

968

14

0.64

1.37

22

HMU05030

putative hydantoinase A

78,444

785

30

0.54

1.15

23

HMU13940

putative exported protein; COG1464: ABC-type metal ion transport system, periplasmic component/surface antigen [inorganic ion transport and metabolism]

29,374

430

29

0.54

1.15

24

HMU05020

undefined product; COG4647/pfam08882, acetone_carb_G

14,279

213

29

0.49

1.04

25

HMU01190

putative hypothetical glycine-rich autotransporter protein

194,616

1,281

19

0.48

1.02

26

HMU00600

putative LPXTG surface protein

133,006

651

14

0.47

1

27

HMU09610

secG putative protein-export membrane protein

12,734

81

21

0.46

0.98

28

HMU12860

sodB superoxide dismutase (Fe)

24,576

231

23

0.46

0.98

29

HMU14090

putative thioredoxin

11,796

138

30

0.46

0.98

30

HMU11370

undefined product; no putative conserved domains detected, hypothetical protein Abu_2077 [Arcobacter butzleri RM4018]: 37%

7,677

92

26

0.46

0.98

31

HMU05010

putative hydantoin hydantoinase A

70,769

835

31

0.45

0.96

32

HMU10410

rpsH 30S ribosomal protein S8

14,807

247

32

0.43

0.91

33

HMU10080

putative putative membrane protein

10,902

103

17

0.42

0.89

34

HMU04360

atpC ATP synthase F1 sector epsilon subunit

13,211

108

16

0.42

0.89

35

HMU04350

atpD ATP synthase F1 sector beta subunit

51,310

581

23

0.42

0.89

36

HMU10380

rpsE 30S ribosomal protein S5

15,592

199

33

0.42

0.89

37

HMU09160

undefined product; no putative conserved hits detected, hypothetical protein HH1743 [Helicobacter hepaticus ATCC 51449]: 32% ID

43,936

335

14

0.40

0.85

38

HMU10290

rpoA DNA-directed RNA polymerase alpha chain

37,551

320

24

0.38

0.82

39

HMU05040

putative hydantoin utilization protein B

83,272

514

23

0.37

0.8

40

HMU05840

putative flagellin

53,982

501

27

0.37

0.79

41

HMU10120

peb4[1]f2 major antigenic peptide PEB3ll binding factor 2

31,816

371

32

0.35

0.76

42

HMU04050

petC putative ubiquinol-cytochrome C reductase cytochrome C subunit

33,425

299

25

0.35

0.74

43

HMU04380

exbD3 exbD olR family transport protein

14,662

132

23

0.35

0.74

44

HMU06080

hypothetical protein Cj0372; COG0754/pfarm03738: Glutathionylspermidine synthase [amino acid transport and metabolism]

44,843

197

12

0.35

0.74

45

HMU11040

fliL possible flagellar protein

18,741

175

18

0.33

0.7

46

HMU04330

atpA ATP synthase F1 sector alpha subunit

55,012

371

20

0.31

0.67

47

HMU07380

hupB DNA-binding protein HU homolog

10,122

142

26

0.29

0.61

48

HMU03350

rplS 50S ribosomal protein L19

13,546

226

28

0.29

0.61

49

HMU03180

rplL 50S ribosomal protein L7/L12

12,946

97

18

0.27

0.58

50

HMU01920

htrA serine protease (protease DO)

45,914

305

17

0.27

0.58


The 50 most abundant proteins are tabulated.

a. Relative abundance ranked by mol%

b. Score for the entire protein derived by MASCOT, and made up of the individual scores given to each peptide sequence

c. Proportion of each protein sequence identified

d. Exponentially modified protein abundance index. See Methods for details and reference.

O'Toole et al. BMC Genomics 2010 11:164   doi:10.1186/1471-2164-11-164

Open Data