Schematic overview of a typical BSA expression profiling experiment targeting tuber flesh color as an example. Extreme individuals from a segregating population are identified and pools of RNA are collected. Gene expression is profiled for each of the pools using microarray technology. Genes displaying differential expression between the contrasting bulks are considered as candidate genes and further analyzed targeting the individual genotypes.
Kloosterman et al. BMC Genomics 2010 11:158 doi:10.1186/1471-2164-11-158