Table 3

Genes containing uAUGs predicted to interact with expressed miRNAs

Gene

uAUG

Cell line used in experiments

miRNAs predicted to interact§

Evidence of miRNA expression


MOR

gcccAUGcucc (1×)

HEK293

hsa-miR-146a (3')

No

hsa-miR-202 (3')

No

ggggAUGcuaa (2×)

hsa-miR-324-5p (5')

Yes[42]

hsa-miR-517b (5')

Yes[42]

aaggAUGcgcc (3×)

hsa-miR-323-5p (3')

No

hsa-miR-324-5p (5')

Yes[42]

hsa-miR-450b-3p (3')

No


CHOP

uaucAUGuuaa (1×)

HeLa

None

aaagAUGagcg (6×)

hsa-miR-574-3p (5')

Yes[41,42]

hsa-miR-556-3p (3')

No

gcagAUGugcu (2×)

hsa-miR-219-2-3p (3')

No*


MDM2

aaagAUGgagc (3×)

HeLa

hsa-miR-363 (3')

Yes[42]

uggaAUGaucc (1×)

None


ADH5/FDH

gcccAUGccuc (4×)

HeLa

hsa-miR-146a (3')

No

hsa-miR-202 (3')

No

ccggAUGucag (4×)

hsa-miR-219-1-3p (3')

No*

hsa-miR-219-2-3p (3')

No*

hsa-miR-487a (3')

No

hsa-miR-489 (5')

No


KLF13

cacaAUGcgcg# (1×)

Jurkat

hsa-miR-323-5p (3')

No

hsa-miR-103 (5')

Yes[41]

hsa-miR-107 (5')

Yes[41]

hsa-miR-33a (5')

Yes[44,45]

ccccAUGcgcu (2×)

hsa-miR-586 (5')

No

hsa-miR-202 (3')

No

gcggAUGcgcg (2×)

hsa-miR-450b-3p (3')

No

hsa-miR-324-5p (5')

Yes[41,44]


uAUGs shown in caps. Strength of translation repression (1×-6×) was deduced by comparing the effect of the uAUG either to a control construct or to a construct where the uAUG under consideration is mutated or deleted.

# uAUG not present in the GenBank entry but used in reporter constructs [26].

§ Numbers in parentheses indicate the miRNA end predicted to interact. miRNAs in italics indicate matches with one GU wobble.

Reference for evidence of expression.

*Expression of mature miR-219, which corresponds to the 5p arm of the precursor, was detected by Chen et al., but that of 3p was not assayed for on the microarray [42].

Ajay et al. BMC Genomics 2010 11:155   doi:10.1186/1471-2164-11-155

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